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- Characterisation of monoclonal antibodies directed against the N-terminus of human mu and delta opioid receptors
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Characterisation of monoclonal antibodies directed against the N-terminus of human mu and delta opioid receptors
Abstract
Opioid receptors play an important role in the control of nociceptive pathways. They are involved in drug dependence as well as in a number of pathologies related to pain and inflammation. A better understanding of the underlying cellular and molecular mechanisms constitutes therefore a critical issue to decipher the role of opioid receptors. Such studies often require to acknowledge the presence of the receptor or to localize it within a cell or a tissue. In this context we generated monoclonal antibodies directed against the amino-terminus of human mu and delta opioid receptors. In immunofluorescence experiments, the antibodies detected recombinant opioid receptors expressed in baculovirus-infected insect cells or mammalian cells as well as endogenous opioid receptors expressed in a human neuroblastoma cell line. Also they immunoprecipitated the unglycosylated form of recombinant opioid receptors. Finally anti-mu opioid receptor antibodies could be used to identify non glycosylated receptor in Western blotting experiments.
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About: Dominique Massotte
Département de Neurobiologie, UMR 7104, Institut de Génétique et Biologie Moléculaire et Cellulaire (IGBMC), 1 rue Laurent Fries, BP 10142, F-67404 Illkich cedex, France, massotte@igbmc.u-strasbg.fr