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S. SCHOONBROODT, Jacques Piette & Bernard RENTIER

ÉTUDE ULTRASTRUCTURALE DE L'ORGANISATION DU NUCLÉOIDE DU VIRUS DE LA VARICELLE ET DU ZONA
ULTRASTRUCTURAL STUDY OF THE ORGANIZATION OF VARICELLA-ZOSTER VIRUS NUCLEOID

(Volume 60 - Année 1991 — Numéro 6)
Article
Open Access
Mots-clés : electron microscopy, virus morphology.

Abstract

Varicella-Zoster Virus belongs to the alpha-Herpesvirus family.  The morphological characteristics of this group are a central core made of DNA and proteins, an icosahedral capsid, a granular material called tegument and an envelope. The aim of this work was to determine the conditions under which virus morphology appears best preserved.  The preparation conditions have been optimized using a panel of fixing agents and dehydratants.  We have demonstrated that methanol, DMSO and ethanol/methanol allowed a better preservation of the viral particles and could be determinant for the appearance of the core.  Methanol appears as the best because it permits the observation of more complex internal VZV nucleoid organization.

To cite this article

S. SCHOONBROODT, Jacques Piette & Bernard RENTIER, «ÉTUDE ULTRASTRUCTURALE DE L'ORGANISATION DU NUCLÉOIDE DU VIRUS DE LA VARICELLE ET DU ZONA», Bulletin de la Société Royale des Sciences de Liège [En ligne], Volume 60 - Année 1991, Numéro 6, 373 - 383 URL : https://popups.uliege.be/0037-9565/index.php?id=1507.

About:  S. SCHOONBROODT

Secteur de Virologie. Département de Microbiologie, B23 Sart-Tilman, Université de Liège, B-4000 Liège (Belgique)

About: Jacques Piette

Secteur de Virologie. Département de Microbiologie, B23 Sart-Tilman, Université de Liège, B-4000 Liège (Belgique)

About: Bernard RENTIER

Secteur de Virologie. Département de Microbiologie, B23 Sart-Tilman, Université de Liège, B-4000 Liège (Belgique)