Acta Stereologica Acta Stereologica -  Volume 12 (1993)  Number 2 - Proceedings of the sixth European congress for stereology - Part one - Dec. 1993 

Number and size distribution of cerebellar neurons estimated by the optical fractionator and the vertical rotator

Jytte Overgaard Larsen, 
Stereological Research Laboratory, Institute of Experimental Clinical Research, University Institute of Pathology and Second University Clinic of Internal Medicine, Department of Neurology, University of Aarhus, Denmark
Trine Tandrup, 
Stereological Research Laboratory, Institute of Experimental Clinical Research, University Institute of Pathology and Second University Clinic of Internal Medicine, Department of Neurology, University of Aarhus, Denmark
Hans Brændgaard, 
Stereological Research Laboratory, Institute of Experimental Clinical Research, University Institute of Pathology and Second University Clinic of Internal Medicine, Department of Neurology, University of Aarhus, Denmark

Abstract

This study describes how two recently developed design based stereological methods can be used to obtain estimates of the number and size of Purkinje cells and granule cells in rat cerebellum: the optical fractionator has been used to count the cells and the vertical rotator has been used to make estimates of the volumes of the Purkinje cells. Both techniques were highly efficient in that the coefficients of error (CEs) of the individual estimates of the total numbers of Purkinje cells and granule cells were about 10 % when 100 cells of each type were counted in each rat and the CEs of the individual estimates of mean cellular volumes were 3-4 % when ~ 100 Purkinje cells were analysed with the rotator.

Keywords : mean volume, number, optical fractionator, rat cerebellum, size distribution, vertical rotator

Pour citer cet article

Jytte Overgaard Larsen, Trine Tandrup & Hans Brændgaard, «Number and size distribution of cerebellar neurons estimated by the optical fractionator and the vertical rotator», Acta Stereologica [En ligne], Volume 12 (1993), Number 2 - Proceedings of the sixth European congress for stereology - Part one - Dec. 1993, 283-288 URL : https://popups.uliege.be/0351-580x/index.php?id=1664.