Correction for autoradiographic image spread: a comparison between two methods
Abstract
A set of LM autoradiographic images of 125I-insulin attached to the insulin receptors of free rat hepatocytes have been analysed by two methods incorporating correction for image spread effects. Results obtained by an unrestricted analysis based on the construction and use of crossfire matrices are compared with those obtained using an alternative method more recently proposed for populations of isolated radioactive sources of well-defined shape. In the latter approach, the cell profiles were modelled as radioactive discs with superimposed labelled annuli. Both methods indicate significant peripheral labelling, in accordance with current views on the behaviour of insulin receptors, but quantitative estimates of the ratio of interior to peripheral radioactive concentrations are somewhat disparate. Some possible explanations for this are suggested.
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