Image analysis and the determination of codistribution and coexistence of neuroactive substances in nerve terminal populations
Kjell Fuxe,
Department of Histology, Karolinska Institutet, Stockholm, Sweden
Luigi F. Agnati,
Department of Human Physiology, University of Modena, Modena, Italy
Anders Härfstrand,
Department of Histology, Karolinska Institutet, Stockholm, Sweden
Michele Zoli,
Department of Human Physiology, University of Modena, Modena, Italy
Ann Marie Jansson,
Department of Histology, Karolinska Institutet, Stockholm, Sweden
Abstract
The section method of determining coexistence in nerve terminal populations using automatic image analysis and the occlusion principle is described. By means of the dilatation function of the IBAS image analyzer an index has been developed for the description of the density and/or eveness of nerve terminal networks. Furthermore, by means of a logical operation "and" codistribution of densely packed nerve terminals can be determined based on the field area of the overlap image automatically determined in the image analysis. This procedure has been used in the analysis of codistribution of transmitter identified nerve terminals in the median eminence.
Keywords : codistribution, coexistence, image analysis, nerve terminals
Pour citer cet article
Kjell Fuxe, Luigi F. Agnati, Anders Härfstrand, Michele Zoli & Ann Marie Jansson, «Image analysis and the determination of codistribution and coexistence of neuroactive substances in nerve terminal populations», Acta Stereologica [En ligne], Volume 4 (1985), Number 2 - Proceedings of the fourth European symposium for stereology - Dec. 1985, 181-186 URL : https://popups.uliege.be/0351-580x/index.php?id=3159.