Grading of postcapillary venules in lymph nodes: observer variation and reproducibility in subjective estimation and morphometric measurements
Veli-Matti Kosma,
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Kalevi Selkäinaho,
Department of Applied Mathematics, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Yrjö Collan,
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Kari Syrjänen,
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Marja-Leena Aalto,
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Anders Seppä,
Department of Pathology, University of Kuopio, P.O.Box 6, SF 70211 Kuopio 21, Finland
Abstract
We wanted to estimate variation and reproducibility in grading and in morphometric measurements of postcapillary venules. Five pathologists measured 100 postcapillary venules from 20 axillary lymph nodes by subjective and morphometric means. Range of variation was similar in subjective and morphometric measurements. The correlation coefficients between subjective and morphometric measurements varied from 0.62 to 0.93. On subjective grading of the individual postcapillary venules the kappa coefficient was 0.50 (90% limits: 0.40-0.56). On morphometry ICC was 0.74 (0.70-0.78). On morphometric measurements of individual lymph nodes ICC was 0.82. The results show that grading and morphometric measurements are very similar in respect to variability and reproducibility in this kind of experiment.
Pour citer cet article
Veli-Matti Kosma, Kalevi Selkäinaho, Yrjö Collan, Kari Syrjänen, Marja-Leena Aalto & Anders Seppä, «Grading of postcapillary venules in lymph nodes: observer variation and reproducibility in subjective estimation and morphometric measurements», Acta Stereologica [En ligne], Volume 3 (1984), Number 1 - May 1984, 39-42 URL : https://popups.uliege.be/0351-580x/index.php?id=3921.
