Morphometry of connective tissue in rat livers
Otto Löw,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Ulrich Gabler,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Andreas Müller,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Petra Gabler,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Harald Schubert,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Hans-Christoph Löw,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Georg Machnik,
Friedrich-Schiller-University Jena, Klinikum, Bachstr. 18, D-07740 Jena, Germany
Abstract
Thioacetamide (TAA) induced fibrosis in the rat liver was used as a model for testing preparations for a prevention of cirrhosis. The homogeneity of collagen distribution between the lobes allows the morphometry of a single lobe as a measure for the total organ, but it is always better to estimate about 5 stage sections with equal distances, and these sections should be measured totally.
A test period of 3 months results in a markable fibrosis, sufficient for testing antifibrotic preparations.
Zinc ions in drinking water decrease the collagen percentages in comparison to untreated animals and reduce the TAA-induced fibrosis during simultaneously application over a 3 month period, since an addition only in the 3rd month diminishes this effect. No differences are seen after an interruption of the TAA-poisoning after 3 months and a feeding with zinc sulphate only in the 4th month, compared to clear drinking water.
Keywords : connective tissue, methodology, rat liver, thioacetamide, zinc sulphate
Pour citer cet article
Otto Löw, Ulrich Gabler, Andreas Müller, Petra Gabler, Harald Schubert, Hans-Christoph Löw & Georg Machnik, «Morphometry of connective tissue in rat livers», Acta Stereologica [En ligne], Volume 13 (1994), Number 1 - Proceedings of the sixth European congress for stereology - Part two - May 1994, 101-106 URL : https://popups.uliege.be/0351-580x/index.php?id=4533.
