The number of metaphasic granulosa cells in isolated ovarian follicles estimated using the nucleator principle
Peter Valdemar Bagger,
Laboratory of Reproductive Biology, Department of Obstetrics and Gynecology, Rigshospitalet, University of Copenhagen, DK-2100, Copenhagen, Denmark
Morten Dyrved Christiansen,
Laboratory of Reproductive Biology, Department of Obstetrics and Gynecology, Rigshospitalet, University of Copenhagen, DK-2100, Copenhagen, Denmark
Hans Jørgen G. Gundersen,
Stereological Research Laboratory, Bartholin Building, Aarhus University, DK-8000 Århus C, Denmark
Abstract
Nine murine ovarian follicles embedded in plastic were orientated isotropically using the Orientator by Mattfeldt, cut into 20µm serial sections and stained with hematoxylin. The number of granulosa cells in metaphase in each follicle was estimated using the Nucleator principle in the section which contained the nucleolus of the oocyte. The true number of granulosa cells in metaphase was obtained from the complete set of follicle sections using the 3-D counting rule. By knowing the truth, the precision (CE) of the Nucleator estimates could be calculated. The Nucleator provides sufficiently precise estimates of the small fraction of metaphasic granulosa cells. The estimates are unbiased and expressed in 3-D terms, thus making them suitable for statistical analysis and straight-forward biological interpretation.
Keywords : Disector, follicle, granulosa cells, Nucleator, stereology
Pour citer cet article
Peter Valdemar Bagger, Morten Dyrved Christiansen & Hans Jørgen G. Gundersen, «The number of metaphasic granulosa cells in isolated ovarian follicles estimated using the nucleator principle», Acta Stereologica [En ligne], Volume 13 (1994), Number 1 - Proceedings of the sixth European congress for stereology - Part two - May 1994, 83-88 URL : https://popups.uliege.be/0351-580x/index.php?id=4588.
