Quantification of three-dimensional vascular patterns in renal glomeruli
Elźbieta Kaczmarek,
Department of Cellular Pathology, Armed Forces Institute of Pathology, Washington, D.C. 20306-6000, USA
Abstract
A method is developed for quantitative characterization of 3D capillary networks. Volume data was obtained by stacking up confocal epifluorescent images of renal glomeruli with a Zeiss CLSM. To provide the 3D data set, a region of interest containing an entire glomerulus was extracted from the volume data. A reconstruction of a capillary network with nodes representing transversal profiles of capillary lumens is made by applying a node-branch model of 3D vascular patterns. This network is used to derive the connectivity of the 3D vascular structure and the length and number of capillaries.
Keywords : confocal microscopy, image processing, capillaries, glomerulus, 3D reconstruction
Pour citer cet article
Elźbieta Kaczmarek, «Quantification of three-dimensional vascular patterns in renal glomeruli», Acta Stereologica [En ligne], Volume 15 (1996), Number 2 - Applications of stereology in life sciences - July 1996, 147-152 URL : https://popups.uliege.be/0351-580x/index.php?id=548.