Automatic detection of sphere distribution in thick section
Kensuke Baba,
Department of Pathology and Surgical Pathology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807, Japan
Kiyoshi Miyamoto,
Institute of Medical Sciences, Dokkyo University School of Medicine, Japan
Kazumonto Kimura,
Institute of Medical Sciences, Dokkyo University School of Medicine, Japan
Hidetaka Matsukuma,
Department of Pathology and Surgical Pathology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807, Japan
Osamu Koide,
Department of Pathology and Surgical Pathology, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi, Kitakyushu, Fukuoka 807, Japan
Abstract
The size distribution of somatotroph secretory granules on electronmicrographes of rat adenohypophysis is automatically detected by two methods; 1) "erosion-chord method" and 2) "distance-maximum method". In the later method, the diameter of each disc of the granules is automatically detected in a way which depends upon Rosenfeld's distance function. In the former method, the Serra's erosion is employed prior to the detection of the chord length to separate the profile of the granules on the electron micrographs. The two results are compared with the results using the manual methods. In these analyses, the thickness of the section is stereologically introduced.
Pour citer cet article
Kensuke Baba, Kiyoshi Miyamoto, Kazumonto Kimura, Hidetaka Matsukuma & Osamu Koide, «Automatic detection of sphere distribution in thick section», Acta Stereologica [En ligne], Volume 3 (1984), Number 1 - May 1984, 57-62 URL : https://popups.uliege.be/0351-580x/index.php?id=3945.