A three dimensional quantitative approach for colocalization study of cyclin A and P34cdk1 by confocal microscopy
Richard Delorme,
Laboratoire de Cytologie Analytique, Université Claude-Bernard Lyoni, 8 avenue Rockefeller, 69373 Lyon CEDEX 08, France
Catherine Souchier,
Laboratoire de Cytologie Analytique, Université Claude-Bernard Lyoni, 8 avenue Rockefeller, 69373 Lyon CEDEX 08, France
Paul-André Bryon,
Laboratoire de Cytologie Analytique, Université Claude-Bernard Lyoni, 8 avenue Rockefeller, 69373 Lyon CEDEX 08, France
Abstract
In mammalian cells, cyclin A and cyclin B are known to regulate P34cdkl activity during the entry into mitosis, whereas cyclin A also regulates p33cdk2 kinase. Confocal laser scanning microscopy acquisition and three dimensional image analysis were performed to quantitatively estimate cyclin A and P34cdk1 colocalization on lymphoid RAMOS cell line. Cytofluorograms were used to derive parameters estimating colocalization. Colocalization globally increased when cells accumulated cyclin A. However, results demonstrated that some positive voxels were not colocalized.
Keywords : colocalization, confocal microscopy, cyclin A, double labelling, image analysis, P34cdk1
Pour citer cet article
Richard Delorme, Catherine Souchier & Paul-André Bryon, «A three dimensional quantitative approach for colocalization study of cyclin A and P34cdk1 by confocal microscopy», Acta Stereologica [En ligne], Volume 14 (1995), Number 2 - Quantitative image analysis - Nov. 1995, 155-160 URL : https://popups.uliege.be/0351-580x/index.php?id=816.