Acta Stereologica

0351-580X

 

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Leszek Cieciura, Dariusz Tosik & Krzysztof Zieliński

Changes in freeze-fractured mitochondrial membranes during spermatogenesis

(Volume 10 (1991) — Number 1 - Stermat '90 (part I) - June 1991)
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Abstract

The mitochondria of germ cells in spermatogenesis, and the transformation of mitochondria from an orthodox form in spermatogonia to a condensed form in spermatids has been described and widely characterized by TEM. The morphological correlation of freeze-fractured mitochondrial membranes with the energetic states of mitochondria has also been described. The frequency by which the fracture plane following the inner or outer boundary membrane deviates by jumping from one to the other is higher in phosphorylating mitochondria than in non-phosphorylating mitochondria. The present study on replicas of freeze-fractured spermatogenic cells of the rat showed that the frequency of deflections of the fracture plane was lowest in spermatogonial mitochondria and increased in spermatocytes and spermatids. Our study seems to suggest that this method can be applied to tissue samples also after glutaraldehyde fixation.

Keywords : freeze-fractured mitochondrial membranes, spermatogenesis

To cite this article

Leszek Cieciura, Dariusz Tosik & Krzysztof Zieliński, «Changes in freeze-fractured mitochondrial membranes during spermatogenesis», Acta Stereologica [En ligne], Volume 10 (1991), Number 1 - Stermat '90 (part I) - June 1991, 131-136 URL : https://popups.uliege.be/0351-580x/index.php?id=2124.

About: Leszek Cieciura

Department of Histology and Embryology MMA, Plac Hallera 1, 90-647 Łódź, Poland

About: Dariusz Tosik

Department of Histology and Embryology MMA, Plac Hallera 1, 90-647 Łódź, Poland

About: Krzysztof Zieliński

Department of Histology and Embryology MMA, Plac Hallera 1, 90-647 Łódź, Poland