Acta Stereologica

0351-580X

 

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Richard E. Coggeshall

On numerical estimates from histological material: the question of calibration

(Volume 18 (1999) — Number 2 - July 1999)
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Abstract

The present work concerns the evaluation of various types of methods for determining numerical data from histologic sections. Major points are:

1. Simple profile counts or estimates from assumption based methods require validation by calibration if the estimates are to be accepted.

2. Calibration is thus a useful thing, but it should be understood that one is not calibrating methods; one is determining whether particular estimates from any particular method are unbiased.

3. Whenever conditions change, assumption based estimates must be recalibrated.

4. Since calibration requires comparison against unbiased estimates, it is usually simpler to use the unbiased estimates.

Keywords : assumption based methods, stereology

Om dit artikel te citeren:

Richard E. Coggeshall, «On numerical estimates from histological material: the question of calibration», Acta Stereologica [En ligne], Volume 18 (1999), Number 2 - July 1999, 219-224 URL : https://popups.uliege.be/0351-580x/index.php?id=206.

Over : Richard E. Coggeshall

Department of Anatomy & Neurosciences, Marine Biomedical Institute, The University of Texas Medical Branch, Galveston, Texas 77555-1069