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- Volume 4 (1985)
- Number 1 - Nov. 1985
- Morphometric analysis of normal muscle biopsies: a statistical approach
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Morphometric analysis of normal muscle biopsies: a statistical approach
Abstract
With a Quantimet 720, an automated image analysis of muscle cross-sections has been developed, using the property of muscle tissue to display two main fibre types with the ATPase reaction. 14 parameters, including size, number and spatial, distribution, have been investigated on 53 biopsies of normal leg muscles. The data were tested in relation with sex and increasing age. Some trends are apparent which indicate a certain wide variability within the morphometric parameters concerning the normal muscle. A principal component analysis revealed 3 distinct groups of parameters. The most reliable ones are the type I/type II area ratio and the coefficient of variation of the fibre size.